Tea tree oil nanostructured lipid carrier

Excerpt:


J. Pharm. Pharmacogn. Res., vol. 11, no. 3, pp. 391-400, May-June 2023. DOI: https://doi.org/10.56499/jppres23.1581_11.3.391 Original Article Development of nanostructured lipid carrier containing tea tree oil: Physicochemical properties and stability [Desarrollo de un portador lipídico nanoestructurado que contiene aceite del árbol del té: Propiedades fisicoquímicas y estabilidad] Endang Wahyu Fitriani1,2, Christina Avanti2, Yeva Rosana3, Silvia Surini1* 1Laboratory … Continue reading Tea tree oil nanostructured lipid carrier

J. Pharm. Pharmacogn. Res., vol. 11, no. 3, pp. 391-400, May-June 2023.

DOI: https://doi.org/10.56499/jppres23.1581_11.3.391

Original Article

Development of nanostructured lipid carrier containing tea tree oil: Physicochemical properties and stability

[Desarrollo de un portador lipídico nanoestructurado que contiene aceite del árbol del té: Propiedades fisicoquímicas y estabilidad]

Endang Wahyu Fitriani1,2, Christina Avanti2, Yeva Rosana3, Silvia Surini1*

1Laboratory of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.

2Laboratory of Pharmaceutics, Faculty of Pharmacy, University of Surabaya, Surabaya, 60293, Indonesia.

3Clinical Microbiology Laboratory, Faculty of Medicine, Universitas Indonesia - Ciptomangunkusumo Hospital, Jakarta, Indonesia.

*E-mail: silvia@farmasi.ui.ac.id

Abstract

Context: Tea tree oil (TTO) is an essential oil derived from Melaleuca alternifolia, with high antimicrobial and antifungal potential. Unfortunately, its topical antifungal efficacy is limited because it is volatile, thermolabile and easily oxidized. A formulation has been developed to overcome this problem by encapsulating TTO in a nanostructured lipid carrier (NLC).

Aims: To determine the effect of the liquid to solid lipid ratio on the physicochemical properties and the stability of TTO-loaded NLC.

Methods: Five formula of TTO-loaded NLCs were produced by high shear homogenization method and characterized according to their particle size, size distribution, polydispersity, zeta potential, thermal characteristics, X-ray diffraction, and terpinen-4-ol concentration. In addition, a stability study was conducted by observing its physical and chemical characteristics during storage in the refrigerator (4 ± 2°C) and at room temperature (27 ± 2°C) for six months.

Results: The resulting TTO-loaded NLC had an average droplet size under 400 nm. The particle size increases with increasing amount of liquid lipid in the formula. There were insignificant changes in organoleptic properties, polydispersity index, zeta potential and terpinene-4-ol concentration during stability study for six months. However, the particle size slightly increased during the six months of storage. Furthermore, the NLC 3, which formulated with a 25:95 ratio liquid to solid lipid, was be chosen as the best formula, since it demonstrated the best physicochemical characteristic and stability.

Conclusions: TTO-loaded NLC with good physicochemical characteristics and stability has been successfully developed. In addition, NLC 3 is considered as the best NLC formula, which exhibits characteristics and stability that meet the requirements.

Keywords: differential scanning calorimetry; nanostructured lipid carrier; physicochemical stability; tea tree oil; terpinen-4-ol.

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Resumen

Contexto: El aceite del árbol del té (TTO) es un aceite esencial derivado de Melaleuca alternifolia, con un alto potencial antimicrobiano y antifúngico. Desgraciadamente, su eficacia antifúngica tópica es limitada porque es volátil, termolábil y se oxida fácilmente. Se ha desarrollado una formulación para superar este problema encapsulando TTO en un portador lipídico nanoestructurado (NLC).

Objetivos: Determinar el efecto de la proporción entre lípidos líquidos y sólidos sobre las propiedades fisicoquímicas y la estabilidad del NLC cargado con TTO.

Métodos: Se produjeron cinco fórmulas de NLC cargadas con TTO mediante el método de homogeneización de alto cizallamiento y se caracterizaron según su tamaño de partícula, distribución de tamaño, polidispersidad, potencial zeta, características térmicas, difracción de rayos X y concentración de terpinen-4-ol. Además, se realizó un estudio de estabilidad observando sus características físicas y químicas durante su almacenamiento en el frigorífico (4 ± 2°C) y a temperatura ambiente (27 ± 2°C) durante seis meses.

Resultados: La NLC cargada con TTO resultante tenía un tamaño medio de gota inferior a 400 nm. El tamaño de partícula aumenta con el incremento de la cantidad de lípido líquido en la fórmula. Se produjeron cambios insignificantes en las propiedades organolépticas, el índice de polidispersidad, el potencial zeta y la concentración de terpineno-4-ol durante el estudio de estabilidad durante seis meses. Sin embargo, el tamaño de las partículas aumentó ligeramente durante los seis meses de almacenamiento. Además, la NLC 3, formulada con una proporción 25:95 de lípidos líquidos y sólidos, fue elegida como la mejor fórmula, ya que demostró las mejores características fisicoquímicas y de estabilidad.

Conclusiones: Se ha desarrollado con éxito un NLC cargado con TTO con buenas características fisicoquímicas y estabilidad. Además, la NLC 3 se considera la mejor fórmula de NLC, que presenta características y estabilidad que cumplen los requisitos.

Palabras Clave: aceite del árbol del té; calorimetría diferencial de barrido; estabilidad fisicoquímica; portador lipídico nanoestructurado; terpinen-4-ol.

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Citation Format: Fitriani EW, Avanti C, Rosana Y, Surini S (2023) Development of nanostructured lipid carrier containing tea tree oil: Physicochemical properties and stability. J Pharm Pharmacogn Res 11(3): 391–400. https://doi.org/10.56499/jppres23.1581_11.3.391
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