{"id":14051,"date":"2023-01-02T12:58:55","date_gmt":"2023-01-02T15:58:55","guid":{"rendered":"https:\/\/jppres.com\/jppres\/?p=14051"},"modified":"2023-01-15T22:42:16","modified_gmt":"2023-01-16T01:42:16","slug":"targeted-proteins-for-vaginal-epithelial-repair","status":"publish","type":"post","link":"https:\/\/jppres.com\/jppres\/targeted-proteins-for-vaginal-epithelial-repair\/","title":{"rendered":"Targeted proteins for vaginal epithelial repair"},"content":{"rendered":"\n

J. Pharm. Pharmacogn.\u00a0Res., vol. 11, no. 1, pp. 110-116, January-February 2023<\/strong>.<\/p>\n\n\n\n

DOI: https:\/\/doi.org\/10.56499\/jppres22.1512_11.1.110<\/a><\/p>\n\n\n\n

Original Article<\/strong><\/p>\n\n\n\n

Epithelial thinning in vaginal atrophy related to lowering of calcitonin gene-related protein, vascular endothelial growth factor, and nerve growth factor expressions in a menopausal rat model<\/h1>\n\n\n\n

[Adelgazamiento epitelial en la atrofia vaginal relacionado con la disminuci\u00f3n de las expresiones de la prote\u00edna relacionada con el gen de la calcitonina, el factor de crecimiento endotelial vascular y el factor de crecimiento nervioso en un modelo de rata menop\u00e1usica]<\/h2>\n\n\n\n

An Nisa Fithri1,2*<\/sup>, Yuyun Yueniwati3<\/sup>, I Wayan Arsana4<\/sup>, Husnul Khotimah5<\/sup>, Wiwit Nurwidyaningtyas6<\/sup><\/h3>\n\n\n\n

1<\/sup>Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Brawijaya, Indonesia.<\/p>\n\n\n\n

2<\/sup>Midwifery Program, Sekolah Tinggi Ilmu Kesehatan Kendedes Malang, 65126, Indonesia.<\/p>\n\n\n\n

3<\/sup>Department of Radiology, Public Saiful Anwar Hospital, Malang, Indonesia,<\/p>\n\n\n\n

4<\/sup>Department of Fertility, Endocrinology and Reproduction, Obstetric and Gynecology Laboratory, Public Saiful Anwar Hospital, Faculty of Medicine, Universitas Brawijaya, Indonesia.<\/p>\n\n\n\n

5<\/sup>Department of Pharmacology, Universitas Brawijaya, Indonesia.<\/p>\n\n\n\n

6<\/sup>Department Molecular and Cellular Biology, Sekolah Tinggi Ilmu Kesehatan Kendedes, Malang, 65126, Indonesia. <\/p>\n\n\n\n

*E-mail: teh.nisa1@gmail.com<\/a><\/p>\n\n\n\n

Abstract<\/strong><\/p>\n\n\n\n

Context<\/em>: Vaginal atrophy has been observed as a common sexual problem in post-menopausal women. The targeted protein to counteract menopause problems related to vaginal epithelial thinning is currently a research problem that has not been fully investigated.<\/p>\n\n\n\n

Aims<\/em>: To explore the possible mechanism underlying vaginal atrophy in rat models.<\/p>\n\n\n\n

Methods<\/em>: Following three-week ovariectomy (OVX), Sprague-Dawley female rats were randomly divided into two groups and orally administered estradiol for two weeks in the treated group. In parallel with this, six rats with sham surgery were used as control. Marker-related vaginal atrophy, including calcitonin gene-related protein (CGRP), vascular endothelial growth factor (VEGF), and nerve growth factor (NGF) in the vaginal wall, were compared using immunohistochemistry.<\/p>\n\n\n\n

Results<\/em>: OVX as a menopausal model significantly induced vaginal epithelial cell thinning and decreased the expression of CGRP, VEGF, and NGF compared with sham surgery animals (p<0.05). Estrogen replacement in OVX rats reversed the vaginal atrophic by recovering the protein expression CGRP, VEGF, and NGF (p<0.05). <\/p>\n\n\n\n

Conclusions<\/em>: Thus, it may be concluded that a possible mechanism underlying the OVX-induced vaginal atrophy may be related to the downregulation expression of CGRP, VEGF, and NGF in vaginal tissue.<\/p>\n\n\n\n

Keywords<\/em>: calcitonin gene-related protein; menopause; nerve growth factor; ovariectomy model; vaginal atrophy; vascular endothelial growth factor.<\/p>\n\n\n\n

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Resumen<\/h4>\n\n\n\n

Contexto<\/em>: Se ha observado que la atrofia vaginal es un problema sexual com\u00fan en las mujeres posmenop\u00e1usicas. La prote\u00edna dirigida a contrarrestar los problemas de la menopausia relacionados con el adelgazamiento del epitelio vaginal es actualmente un problema de investigaci\u00f3n que no se ha investigado completamente.<\/p>\n\n\n\n

Objetivos<\/em>: Explorar el posible mecanismo subyacente a la atrofia vaginal en modelos de rata.<\/p>\n\n\n\n

M\u00e9todos:<\/em> Tras una ovariectom\u00eda (OVX) de tres semanas, se dividieron aleatoriamente ratas hembras Sprague-Dawley en dos grupos y se les administr\u00f3 estradiol por v\u00eda oral durante dos semanas en el grupo tratado. Paralelamente, se utilizaron como control seis ratas con cirug\u00eda simulada. Se compararon mediante inmunohistoqu\u00edmica los marcadores de atrofia vaginal, como la prote\u00edna relacionada con el gen de la calcitonina (CGRP), el factor de crecimiento endotelial vascular (VEGF) y el factor de crecimiento nervioso (NGF) en la pared vaginal.<\/p>\n\n\n\n

Resultados<\/em>: La OVX como modelo menop\u00e1usico indujo significativamente el adelgazamiento de las c\u00e9lulas epiteliales vaginales y disminuy\u00f3 la expresi\u00f3n de CGRP, VEGF y NGF en comparaci\u00f3n con los animales sometidos a cirug\u00eda simulada (p<0,05). El reemplazo de estr\u00f3genos en las ratas OVX revirti\u00f3 la atrofia vaginal recuperando la expresi\u00f3n de prote\u00ednas CGRP, VEGF y NGF (p<0,05).<\/p>\n\n\n\n

Conclusiones<\/em>: Por lo tanto, se puede concluir que un posible mecanismo subyacente a la atrofia vaginal inducida por la OVX puede estar relacionado con la disminuci\u00f3n de la expresi\u00f3n de CGRP, VEGF y NGF en el tejido vaginal.<\/p>\n\n\n\n

Palabras Clave<\/em>: atrofia vaginal; factor de crecimiento endotelial vascular; factor de crecimiento nervioso; ; menopausia; modelo de ovariectom\u00eda; prote\u00edna relacionada con el gen de la calcitonina.<\/p>\n\n\n\n

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Download the PDF file .<\/a><\/p>\n<\/object>\n<\/div><\/figure>\n\n\n\n

Citation Format:<\/strong> Fithri AN, Yueniwati Y, Arsana IW, Khotimah H, Nurwidyaningtyas W (2023) Epithelial thinning in vaginal atrophy related to lowering of calcitonin gene-related protein, vascular endothelial growth factor, and nerve growth factor expressions in a menopausal rat model. J Pharm Pharmacogn Res 11(1): 110\u2013116. https:\/\/doi.org\/10.56499\/jppres22.1512_11.1.110<\/a><\/pre>\n\n\n\n
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\u00a9 2023 Journal of Pharmacy & Pharmacognosy Research<\/p>\n","protected":false},"excerpt":{"rendered":"
J. Pharm. Pharmacogn.\u00a0Res., vol. 11, no. 1, pp. 110-116, January-February 2023. DOI: https:\/\/doi.org\/10.56499\/jppres22.1512_11.1.110 Original Article Epithelial thinning in vaginal atrophy related to lowering of calcitonin gene-related protein, vascular endothelial growth factor, and nerve growth factor expressions in a menopausal rat model [Adelgazamiento epitelial en la atrofia vaginal relacionado con la disminuci\u00f3n de las expresiones de … Continue reading Targeted proteins for vaginal epithelial repair<\/span> →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[37],"tags":[360,877,878,879,880,881,882],"class_list":["post-14051","post","type-post","status-publish","format-standard","hentry","category-pharmaceutical-science","tag-article","tag-calcitonin-gene-related-protein","tag-menopause","tag-nerve-growth-factor","tag-ovariectomy-model","tag-vaginal-atrophy","tag-vascular-endothelial-growth-factor"],"brizy_media":[],"_links":{"self":[{"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/posts\/14051","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/comments?post=14051"}],"version-history":[{"count":0,"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/posts\/14051\/revisions"}],"wp:attachment":[{"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/media?parent=14051"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/categories?post=14051"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/tags?post=14051"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}