{"id":19321,"date":"2025-01-13T19:56:34","date_gmt":"2025-01-13T22:56:34","guid":{"rendered":"https:\/\/jppres.com\/jppres\/?p=19321"},"modified":"2025-01-13T19:56:34","modified_gmt":"2025-01-13T22:56:34","slug":"synergistic-effect-of-holothuria-scabra-extract-with-chemotherapeutic-agents","status":"publish","type":"post","link":"https:\/\/jppres.com\/jppres\/synergistic-effect-of-holothuria-scabra-extract-with-chemotherapeutic-agents\/","title":{"rendered":"Synergistic effect of Holothuria scabra extract with chemotherapeutic agents"},"content":{"rendered":"

J. Pharm. Pharmacogn. Res., vol. 13, no. 3, pp. 919-924, May-Jun 2025<\/strong>.<\/p>\r\n\r\n\r\nDOI: https:\/\/doi.org\/10.56499\/jppres24.2069_13.3.919<\/a>\r\n\r\n\r\n

Original Article<\/strong><\/p>\r\n\r\n

Extract of Holothuria scabra <\/i>exhibits synergistic effect with chemotherapeutic agents against breast cancer in vitro<\/i><\/h1>\r\n\r\n

[Extracto de Holothuria scabra<\/i> muestra un efecto sin\u00e9rgico con agentes quimioterap\u00e9uticos contra el c\u00e1ncer de mama in vitro<\/i>]<\/h2>\r\n\r\n

Teresa Liliana Wargasetia1<\/sup>*<\/sup>, Laella Kinghua Liana1<\/sup>, Nashi Widodo2<\/sup>, Yuslinda Annisa2<\/sup>, Feri Eko Hermanto3<\/sup><\/h3>\r\n\r\n

1<\/sup>Faculty of Medicine, Universitas Kristen Maranatha (Maranatha Christian University), Bandung, Indonesia.<\/p>\r\n

2<\/sup>Biology Department, Faculty of Mathematics and Natural Sciences, University of Brawijaya, Malang, Indonesia.<\/p>\r\n

3<\/sup>Faculty of Animal Sciences, Brawijaya University, Malang, Indonesia.<\/p>\r\n

*E-mail: teresa.lw@med.maranatha.edu<\/a><\/p>\r\n\r\n

Abstract<\/strong><\/p>\r\n\r\n

Context<\/em>: Sea cucumbers have been identified as highly potential contenders in the fight against breast cancer.<\/p>\r\n

Aims<\/em>: To investigate the potential of Holothuria scabra<\/em> extract for anticancer against breast cancer cells in vitro<\/em> by investigating its synergistic effects when integrated with frequently used chemotherapeutic medications.<\/p>\r\n

Methods<\/em>: The Combenefit software was employed to assess the human breast cancer cell line T47D viability towards a synergistic combination of H. scabra<\/em> extract and chemotherapeutic medications (cisplatin, doxorubicin, and neratinib). The investigation encompassed assessing the combination by utilizing a cell viability experiment employing a water-soluble tetrazolium-1 reagent. Subsequently, the selective cytotoxic effect was assessed by subjecting the highest cytotoxicity score to TIG-1-20 normal cells.<\/p>\r\n

Results<\/em>: The synergistic model was evaluated by comparing interaction analysis through Loewe, Bliss, and Highest Single Agent (HSA) models. Combinations of H. scabra<\/em> and Cisplatin showed synergistic activity with similar T47D cell viability values. However, the Loewe synergistic model had a higher T47D cell viability value, while the combination of H. scabra<\/em> and neratinib had a higher T47D cell viability value. Moreover, co-administration of H. scabra<\/em> and doxorubicin demonstrated notable efficacy in inhibiting the proliferation of T47D cells while exhibiting no cytotoxic effects on TIG-1-20 cells.<\/p>\r\n

Conclusions<\/em>: Concurrent administration of H. scabra<\/em> extract and doxorubicin exhibits promising synergistic properties in inhibiting the proliferation of T47D breast cancer cells while demonstrating no toxicity towards TIG-1-20 normal cells.<\/p>\r\n

<\/p>\r\n

Keywords<\/em>: chemotherapy; complementary medicine; natural products; sea cucumbers.<\/p>\r\n\r\n

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Resumen<\/h4>\r\n\r\n

Contexto<\/em>: Los pepinos de mar han sido identificados como contendientes de alto potencial en la lucha contra el c\u00e1ncer de mama.<\/p>\r\n

Objetivos<\/em>: Investigar el potencial del extracto de Holothuria scabra<\/em> como anticancer\u00edgeno contra las c\u00e9lulas de c\u00e1ncer de mama in<\/em> vitro<\/em> mediante la investigaci\u00f3n de sus efectos sin\u00e9rgicos cuando se integra con medicamentos quimioterap\u00e9uticos de uso frecuente.<\/p>\r\n

M\u00e9todos:<\/em> Se emple\u00f3 el software Combenefit para evaluar la viabilidad de la l\u00ednea celular de c\u00e1ncer de mama humano T47D frente a una combinaci\u00f3n sin\u00e9rgica de extracto de H. scabra<\/em> y medicamentos quimioterap\u00e9uticos (cisplatino, doxorrubicina y neratinib). La investigaci\u00f3n abarc\u00f3 la evaluaci\u00f3n de la combinaci\u00f3n mediante la utilizaci\u00f3n de un experimento de viabilidad celular empleando reactivo tetrazolio-1 soluble en agua. Posteriormente, se evalu\u00f3 el efecto citot\u00f3xico selectivo sometiendo la puntuaci\u00f3n m\u00e1s alta de citotoxicidad a c\u00e9lulas normales TIG-1-20.<\/p>\r\n

Resultados<\/em>: El modelo sin\u00e9rgico se evalu\u00f3 comparando el an\u00e1lisis de interacci\u00f3n a trav\u00e9s de los modelos Loewe, Bliss y Highest Single Agent (HSA). Las combinaciones de H. scabra<\/em> y cisplatino mostraron una actividad sin\u00e9rgica con valores similares de viabilidad celular T47D. Sin embargo, el modelo sin\u00e9rgico de Loewe tuvo un valor de viabilidad celular T47D m\u00e1s alto, mientras que la combinaci\u00f3n de H. scabra<\/em> y neratinib tuvo un valor de viabilidad celular T47D m\u00e1s alto. Adem\u00e1s, la administraci\u00f3n conjunta de H. scabra<\/em> y doxorrubicina demostr\u00f3 una eficacia notable en la inhibici\u00f3n de la proliferaci\u00f3n de c\u00e9lulas T47D, sin exhibir efectos citot\u00f3xicos sobre las c\u00e9lulas TIG-1-20.<\/p>\r\n

Conclusiones<\/em>: La administraci\u00f3n simult\u00e1nea de extracto de H. scabra<\/em> y doxorrubicina exhibe prometedoras propiedades sin\u00e9rgicas en la inhibici\u00f3n de la proliferaci\u00f3n de c\u00e9lulas de c\u00e1ncer de mama T47D, sin demostrar toxicidad hacia las c\u00e9lulas normales TIG-1-20.<\/p>\r\n

<\/p>\r\n

Palabras Clave<\/em>: medicina complementaria; pepinos de mar; productos naturales; quimioterapia.<\/p>\r\n\r\n

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Citation Format:<\/strong> Wargasetia TL, Liana LK, Widodo N, Annisa Y, Hermanto FE (2025) Extract of Holothuria scabra <\/em>exhibits synergistic effect with chemotherapeutic agents against breast cancer in vitro<\/em>. J Pharm Pharmacogn Res 13(3): 919\u2013924. https:\/\/doi.org\/10.56499\/jppres24.2069_13.3.919<\/a><\/pre>\r\n\r\n
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\u00a9 2025 Journal of Pharmacy & Pharmacognosy Research<\/p>","protected":false},"excerpt":{"rendered":"
J. Pharm. Pharmacogn. Res., vol. 13, no. 3, pp. 919-924, May-Jun 2025. DOI: https:\/\/doi.org\/10.56499\/jppres24.2069_13.3.919 Original Article Extract of Holothuria scabra exhibits synergistic effect with chemotherapeutic agents against breast cancer in vitro [Extracto de Holothuria scabra muestra un efecto sin\u00e9rgico con agentes quimioterap\u00e9uticos contra el c\u00e1ncer de mama in vitro] Teresa Liliana Wargasetia1*, Laella Kinghua Liana1, … Continue reading Synergistic effect of Holothuria scabra extract with chemotherapeutic agents<\/span> →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[37],"tags":[],"class_list":["post-19321","post","type-post","status-publish","format-standard","hentry","category-pharmaceutical-science"],"brizy_media":[],"_links":{"self":[{"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/posts\/19321","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/comments?post=19321"}],"version-history":[{"count":0,"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/posts\/19321\/revisions"}],"wp:attachment":[{"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/media?parent=19321"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/categories?post=19321"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/tags?post=19321"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}