{"id":541,"date":"2014-12-12T02:28:32","date_gmt":"2014-12-12T05:28:32","guid":{"rendered":"https:\/\/jppres.com\/jppres\/?p=541"},"modified":"2024-02-14T02:11:29","modified_gmt":"2024-02-14T05:11:29","slug":"genotoxic-potential-of-thalassia-testudinum-bm-21","status":"publish","type":"post","link":"https:\/\/jppres.com\/jppres\/genotoxic-potential-of-thalassia-testudinum-bm-21\/","title":{"rendered":"Genotoxic potential of Thalassia testudinum (BM-21)"},"content":{"rendered":"
J Pharm Pharmacogn Res 2(6): 183-193, 2014.<\/strong><\/h6>\n

DOI: https:\/\/doi.org\/10.56499\/jppres14.045_2.6.183<\/a><\/p>\n

Original Article<\/h3>\n

Genotoxic potential of BM-21, an aqueous-ethanolic extract from Thalassia testudinum marine<\/i> plant<\/h2>\n

[Potencial genot\u00f3xico del BM-21, un extracto hidroalcoh\u00f3lico obtenido de la planta marina Thalassia testudinum<\/i]<\/i><\/h2>\n

Yadira Ansoara<\/sup>, Silvia D\u00edaz-Lleraa<\/sup>*, Janet Pilotob<\/sup>, Janet Morffia<\/sup>, Yahima Fri\u00f3na<\/sup>, Roberto Men\u00e9ndezc<\/sup>, Miguel David Fern\u00e1ndezc<\/sup>, Idania Rodeiroc<\/sup>*<\/h3>\n
a<\/sup>Department of Pharmacology and Toxicology, Pharmacy and Food Institute, University of Havana, 23rd Ave, 21425, PO Box 13600, Havana, Cuba. <\/span><\/em><\/div>\n
b<\/sup>Genotoxicity Laboratory, Centre for Research and Development of Medicaments, 26th Ave and 51 St., Havana, Cuba. <\/span><\/em><\/div>\n
c<\/sup>Laboratory of Pharmacology, Centre of Marine Bioproducts (CEBIMAR), Loma and 37St, Vedado, PO Box 10400, Havana, Cuba.<\/span><\/em><\/div>\n
*E-mail: silvia.llera@gmail.com<\/a>; idania.rodeiro@infomed.sld.cu<\/a><\/span><\/em><\/div>\n

 <\/p>\n

Abstract<\/h5>\n

Context: BM-21 is a hydro-ethanolic extract obtained from the leaves of Thalassia testudinum<\/em> marine plant, which is rich in polyphenols, and it has demonstrated antioxidant, anti-inflammatory, cytoprotective and neuroprotective properties.<\/span><\/p>\n

Aims: To investigate the genotoxicity potential of BM-21.<\/span><\/p>\n

Methods: Salmonella typhimurium<\/em> Hist. – strains were used in the pointmutation test and Escherichia coli<\/em> cells were used in SOS response test. DNA primary damage was tested in hepatocytes of mice treated with oral dose of the extract (2000 mg\/kg). Bone marrow micronucleus assay was used in mice to detect clastogenic damage while serum from the same animals was used to determine MDA levels in order to find out the influence of BM-21 on lipid peroxidation. Positive and negative controls were included in all experimental series. <\/span><\/p>\n

Results: BM-21 did not increase the frequency of reverse mutations in the Ames test, and it did not induce primary damage in E. coli<\/em>. Comet assay showed that 2 000 mg\/kg of BM-21 induced single strand breaks or alkali-labile sites in the hepatocytes from the treated mice. However, no increase in the micronucleus frequency was observed in mice polychromatic erythrocytes and significantly reduced MDA levels were detected.<\/span><\/p>\n

Conclusions: BM-21 was neither mutagenic nor induces DNA damage to prokaryotic cells. Although, it increased DNA strand breaks in vivo, this one was not translated into clastogenic damage to the whole organism. Results suggested that BM-21 was not mutagenic or genotoxic under our experimental conditions.<\/span><\/p>\n

Keywords: <\/strong>Ames test; Comet assay; genotoxicity; micronucleus assay; SOS Chromotest.<\/p>\n

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Resumen<\/h5>\n

Contexto: BM-21 es un extracto hidro-alcoh\u00f3lico de las hojas de la planta marina Thalassia testudinum<\/em>. Este es rico en polifenoles y ha demostrado propiedades antioxidante, anti-inflamatoria, citoprotectora y neuroprotectora. <\/span><\/p>\n

Objetivos: Investigar el potencial genot\u00f3xico del BM-21. <\/span><\/p>\n

M\u00e9todos: Se usaron cepas mutantes de Salmonella typhimurium<\/em> Hist.- en el ensayo de mutaciones puntuales y c\u00e9lulas de Escherichia coli<\/em> en el ensayo de respuesta a SOS. El da\u00f1o primario al ADN se prob\u00f3 en hepatocitos extra\u00eddos de ratones tratados con el extracto (2000 mg\/kg, p.o.). El ensayo de micron\u00facleos se realiz\u00f3 en m\u00e9dula \u00f3sea de ratones para descubrir da\u00f1os clastog\u00e9nicos. El suero de los mismos animales se us\u00f3 para determinar los niveles de MDA y as\u00ed averiguar la influencia de BM-21 sobre la peroxidaci\u00f3n lip\u00eddica. Se incluyeron controles positivos y negativos en cada serie experimental. <\/span><\/p>\n

Resultados: Los datos mostraron que el BM-21 no aument\u00f3 la frecuencia de reversi\u00f3n de mutaciones en el ensayo de Ames y tampoco indujo da\u00f1o primario a c\u00e9lulas de E. coli<\/em>. El ensayo Cometa demostr\u00f3 que BM-21 (2 000 mg\/kg) introdujo rupturas de simple cadena y sitios l\u00e1biles a \u00e1lcali en los hepatocitos extra\u00eddos de los ratones tratados. Sin embargo, no se observ\u00f3 aumento en la frecuencia de micron\u00facleos en eritrocitos policrom\u00e1ticos de rat\u00f3n y los niveles de MDA se redujeron significativamente. <\/span><\/p>\n

Conclusiones: El BM-21 no fue mutag\u00e9nico ni introdujo da\u00f1o al ADN en c\u00e9lulas procari\u00f3ticas. Aunque el extracto aument\u00f3 el da\u00f1o in vivo a la cadena de ADN, este no se tradujo en da\u00f1os clastog\u00e9nicos al organismo entero. Los resultados sugirieron que BM-21 no fue mutag\u00e9nico ni genot\u00f3xico bajo nuestras condiciones experimentales.<\/span><\/p>\n

Palabras Clave: <\/strong>Ensayo de Ames; ensayo Cometa; ensayo de micron\u00facleos; genotoxicidad; SOS Chromotest.<\/p>\n

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Citation Format:<\/strong> Yadira Ansoar, Silvia D\u00edaz-Llera, Janet Piloto, Janet Morffi, Yahima Fri\u00f3n, Roberto Men\u00e9ndez, Miguel David Fern\u00e1ndez, Idania Rodeiro (2014)\u00a0Genotoxic potential of BM-21, an aqueous-ethanolic extract from Thalassia testudinum<\/em> marine plant. J Pharm Pharmacogn Res 2(6): 183-193. DOI: https:\/\/doi.org\/10.56499\/jppres14.045_2.6.183<\/a><\/span><\/pre>\n
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Ver\u00f3nica Miguel, Jon A. Otero, Borja Barrera, Idania Rodeiro, Julio G. Prieto, Gracia Merino, Ana I. \u00c1lvarez (2015) ABCG2\/BCRP interaction with the sea grass Thalassia testudinum<\/em>. Drug Metabolism and Personalized Therapy. 30(4):251-6. DOI: 10.1515\/dmpt-2015-0013<\/a><\/pre>\n
\u00a9 2014 Journal of Pharmacy & Pharmacognosy Research (JPPRes)<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"
J Pharm Pharmacogn Res 2(6): 183-193, 2014. DOI: https:\/\/doi.org\/10.56499\/jppres14.045_2.6.183 Original Article Genotoxic potential of BM-21, an aqueous-ethanolic extract from Thalassia testudinum marine plant [Potencial genot\u00f3xico del BM-21, un extracto hidroalcoh\u00f3lico obtenido de la planta marina Thalassia testudinum<\/i] Yadira Ansoara, Silvia D\u00edaz-Lleraa*, Janet Pilotob, Janet Morffia, Yahima Fri\u00f3na, Roberto Men\u00e9ndezc, Miguel David Fern\u00e1ndezc, Idania Rodeiroc* aDepartment … Continue reading Genotoxic potential of Thalassia testudinum (BM-21)<\/span> →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[47,9],"tags":[],"class_list":["post-541","post","type-post","status-publish","format-standard","hentry","category-preclinical-research","category-toxicology"],"brizy_media":[],"_links":{"self":[{"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/posts\/541","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/comments?post=541"}],"version-history":[{"count":0,"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/posts\/541\/revisions"}],"wp:attachment":[{"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/media?parent=541"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/categories?post=541"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/jppres.com\/jppres\/wp-json\/wp\/v2\/tags?post=541"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}